Abundance and location of 125-I-salmon calcitonin binding site in rat brain.
نویسندگان
چکیده
منابع مشابه
Two-site immunoradiometric assay of intact salmon calcitonin.
We developed a two-site immunoradiometric assay (IRMA) of salmon calcitonin (SCT) that detects intact SCT(1-32) and not peptide fragments of the hormone. This was accomplished by using monoclonal antibodies prepared against the peptide fragments SCT(1-11) and SCT(11-32). Two antibodies with specificity for each of the peptides were purified from their respective ascites and evaluated in a two-s...
متن کاملIdentification of key components in the irreversibility of salmon calcitonin binding to calcitonin receptors.
This study investigates the poor reversibility of salmon calcitonin (sCT) binding to rat and human calcitonin receptors. Efficacy of CT and analogue peptides in (125)I-sCT binding competition and cAMP assays was compared with the dissociation kinetics of (125)I-labelled peptides. Assessment was performed on cells stably expressing either rat or human calcitonin receptors. Dissociation kinetics ...
متن کاملSalmon calcitonin induced head twitch in the rat.
The intracerebroventricular administration of salmon calcitonin (sCT) to rats induced a dose-dependent increase in the number of head twitches and potentiated such a response elicited by L-5-hydroxytryptophan (L-5-HTP). Subcutaneously administered sCT was ineffective "per se" but could again potentiate the head twitch response elicited by L-5-HTP. Since these data suggest a stimulation of the b...
متن کاملIntrathecal Amylin and Salmon Calcitonin Affect Formalin Induced c-Fos Expression in the Spinal Cord of Rats
Background: Amylin and Salmon Calcitonin belong to the calcitonin family of peptides and have high affinity binding sites in the rat spinal cord. The aim of this study was to characterize receptors for Amylin and Salmon Calcitonin functionally in the spinal cord of rats. We assessed the expression of c-Fos in response to intraplantar formalin in the lumbar regions of the spinal cord in consciou...
متن کاملDown-regulation of rat kidney calcitonin receptors by salmon calcitonin infusion evidenced by autoradiography.
In treating age-related osteoporosis and Paget disease of bone, it is of major importance to avoid an escape phenomenon that would reduce effectiveness of the treatment. The factors involved in the loss of therapeutic efficacy with administration of large pharmacological doses of the hormone require special consideration. Down-regulation of the hormone receptors could account for the escape phe...
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ژورنال
عنوان ژورنال: The Japanese Journal of Pharmacology
سال: 1980
ISSN: 0021-5198,1347-3506
DOI: 10.1254/jjp.30.575